Effect of elongation factor 2 and of adenosine diphosphate-ribosylated elongation factor 2 on translocation
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چکیده
منابع مشابه
Elongation factor 2. Amino acid sequence at the site of adenosine diphosphate ribosylation.
Tryptic digestion of [14C]ADP-ribosylated elongation factor 2 (EF-Z), purified from rat liver, yielded a single radioactive peptide. The pure peptide (62% yield) consists of 15 amino acid residues whose sequence is Phe-Asp-Val-His-Asp-ValThr-Leu-His-Ala-Asp-Ala-Ile-X-Arg; the ADP ribose is linked to the unknown residue, X. X is a weakly basic residue which does not correspond to any amino acid ...
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Despite the fact that ADP-ribosylation of eukaryotic elongation factor 2 (EF2) leads to inhibition of protein synthesis, the mechanism by which ADP-ribosylated EF2 (ADPR•EF2) causes this inhibition remains controversial. Here, we applied modeling approaches to investigate the consequences of various modes of ADPR•EF2 inhibitory actions on the two coupled processes, the polypeptide chain elongat...
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The extent of the transfer of the adenosine 5'-diphosphate ribose (ADPR) moiety of nicotinamide adenine dinucleotide onto elongation factor 2 (EF-2) catalyzed by Pseudomonas aeruginosa exotoxin A (PA-toxin) was dependent upon the presence of a reducing agent, dithiotheritol (DTT). The reaction requires DTT in low concentration (1 to 10 mM) and in the absence of DTT less product, ADPR-EF 2, was ...
متن کاملActivity of protein phosphatases against initiation factor-2 and elongation factor-2.
The protein phosphatases active against phosphorylase a, elongation factor-2 (EF-2) and the alpha-subunit of initiation factor-2 (eIF-2) [eIF-2(alpha P)] were studied in extracts of rabbit reticulocytes. Swiss-mouse 3T3 fibroblasts and rat hepatocytes, by use of the specific phosphatase inhibitors okadaic acid and inhibitor proteins-1 and -2. In all three extracts tested, both phosphatase-1 and...
متن کاملThe life and death of translation elongation factor 2.
eEF2 (eukaryotic elongation factor 2) occupies an essential role in protein synthesis where it catalyses the translocation of the two tRNAs and the mRNA after peptidyl transfer on the 80 S ribosome. Recent crystal structures of eEF2 and the cryo-electron microscopy reconstruction of its 80 S complex now provide a substantial structural framework for dissecting the functional properties of this ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1976
ISSN: 0264-6021
DOI: 10.1042/bj1560015